Curriculum vitae
Maryam Sadri
PERSONAL INFORMATION
Surname: Sadri First name: Maryam Date of birth: 26/04/1962
Place of Birth: Tehran, Iran gender: Female Marital status: Married
Nationality: Iranian Language: Persian, English and Turkish
Phone N: +19404655697, +989124476047
Email: maryam.sadri@modares.ac.ir; sadri.biophys@gmail.com
Website: https://dr-sadri.com
Orchid number: https://orchid.org/0000-0002-8342-9042
EDUCATION
Ph. D in Biophysics, Faculty of Biosciences, Tarbiat Modares University (TMU), Tehran, Iran (2009 – 2014)
Ph. D Thesis Title: Static Magnetic Field effect on Viability, Proliferation rate and Neural Differentiation in Umbilical Cord derived Human Mesenchymal Stem Cells
M.Sc. in Biophysics, International Institute of Biochemistry & Biophysics (IBB), Tehran University, Tehran, Iran, (1993-1998)
M.Sc. Thesis Title: Effect of He-Ne Laser beam on apoptosis induction in the rat macrophages
Bachelor in Cell biology, Tehran University, Tehran, Iran, (1988-1993)
RESEARCH INTERESTS
I have broad interest in stem cell research related to physical conditions Just like magnetic fields, leading to differentiation and its application for Human diseases treatment, Regenerative medicine and Healthcare. Living systems including human brain involve moving charges, so response to magnetic fields. This is one of the great mysteries of live which can result in a safe and reliable way to cure human disorders.
PUBLICATIONS
a: Scientific articles
1. Sadri M., Rabbani O. Kouhian A. 2005 “Study of Laser Effect on Protein Synthesis and apoptosis in Alveolar Macrophages”; Modern Genetics No: 3 (32-38)
2. Sadri M., Rabbani O. Kouhian A. 2006 “Study of Laser on apoptosis Induction in the Alveolar Macrophages”; Kowsar Medical Jurnal vol11 No:2(143-154)
3. Maryam sadri, Parviz Abdolmale, Saeid Abroun, Bahareh Beiki, Fazel S. Samani. 2012 “Investigating the impacts of Static Magnetic Field on cell viability and Cell Cycle Progression in Human Mesenchymal Stem cells”; Iranian Joural of Biomedical engineering, Summer, vol.6, No2, pp: 91-98
4. Sadri M., Abdolmaleki P., Abrun S., Beiki B., Sahraneshin Samani F., 2017 "Static Magnetic Field Effect on Cell Alignment, Growth and Differentiation in Human cord-derived Mesenchymal Stem Cells" Cellular and Molecular Bioengineering, Vol. 10, No.3: 249–262
5. Maryam sadri, Parviz Abdolmaleki, Saeid Abroun, Mehrdad Behmanesh, Bahareh Beiki, 2018. Static Magnetic Field effect on Differentiation in Human Mesenchymal Stem Cells". J Stem Cells Res, Rev & Rep. 5(1): 1025.
b: Translation & Edition:
EXPERiMENTAL ACHIEVEMENTS
Attending the following:
3. Theoretical course: Nanotechnology Equipments; ( 2010 United Nations Association of Iran-Tehran, Tehran Iran)
4. Experimental skill courses; DNA & RNA extraction, Gel Electrophoresis, PCR
(Summer 1995: National Genetic & Biotechnology Research Institute-Tehran)
PROFESSIONAL SKILLS
EXPERIENCES
1. The examination of expansion and differentiation of human umbilical cord blood derived hematopoietic and mesenchymal stem cells as a Research Colleague 2010- 2011, Royan institute
2. Cell cycle & apoptosis analysis using flow cytometry Research Colleague 2011 Royan institute
3. Isolation and expansion of Wharton’s jelly derived mesenchyme stem cells
using an effective, rapid and economical method without any enzymatic treatment, as a Research Colleague 2011 to 2014
Royan cord blood ban
4. Polymerase chain reaction: Rt PCR and Real time PCR experiment as a Research Colleague 2013 to 2015 Tarbiat Modares University
TEACHING EXPERIENCES
University Course Date
Payamenour (Tehran-East) center Biophysics 2017-2018
Karaj Islamic Azad university Radiation Biology 2018 to date
Karaj Islamic Azad university Biophysics 2018 to date
PROFESSIONAL PRESENTATION
Dissertation abstract
Static Magnetic Field effect on Viability, Proliferation rate and Neural Differentiation in Umbilical Cord derived Human Mesenchymal Stem Cells
This investigation was performed to evaluate the impact of static magnetic field up to 24 miliTesla (24mT) impact on the viability percentage and the cell cycle progression in Mesenchymal Stem cells derived from human newborn cords. Cells from the third to fifth passage were harvested and counted to provide 60% confluent monolayers. The samples were then exposed to 6, 12, 18 and 24 mili-Tesla static magnetic field and the resulting samples were compared to the control none exposed cultures.
A significant (P<0.05) reduction of the cell viability due to the exposure of 24 hours of the magnetic field was noted in the time frames of 36 and 48 hours, However, the drop in stem cell viability percentage significantly leveled off in the exposed samples 72 hours after the exposure which may be due to cellular repair and regenerative mechanisms.
Development through the cell-cycle, also verified this finding: G1 phase cell population significantly decreased in the treated and cultured groups compared to the corresponding control samples. The reduced viable cell rates may be due to apoptosis since necrosis was never observed in our experiments.
In our experiments neural differentiation was enhanced in the 18 mT/24h static magnetic field exposed mesenchymal stem cells after 21 days of cultivation in differential medium. Immunocyto-chemical studies confirmed Nestin gene expression in the SMF treated samples. Furthermore, Oct-4, Nanong and Sox2 genes were less expressed while Heage-1 over expressed in the SMF exposed cell cultures which suggested the differentiation inducing effects of SMF exposure. Keywords:
Mesenchymal Stem Cell, Static Magnetic Field, Viability, Neural Differentiation
PERSONAL INFORMATION
Surname: Sadri First name: Maryam Date of birth: 26/04/1962
Place of Birth: Tehran, Iran gender: Female Marital status: Married
Nationality: Iranian Language: Persian, English and Turkish
Phone N: +19404655697, +989124476047
Email: maryam.sadri@modares.ac.ir; sadri.biophys@gmail.com
Website: https://dr-sadri.com
Orchid number: https://orchid.org/0000-0002-8342-9042
EDUCATION
Ph. D in Biophysics, Faculty of Biosciences, Tarbiat Modares University (TMU), Tehran, Iran (2009 – 2014)
Ph. D Thesis Title: Static Magnetic Field effect on Viability, Proliferation rate and Neural Differentiation in Umbilical Cord derived Human Mesenchymal Stem Cells
M.Sc. in Biophysics, International Institute of Biochemistry & Biophysics (IBB), Tehran University, Tehran, Iran, (1993-1998)
M.Sc. Thesis Title: Effect of He-Ne Laser beam on apoptosis induction in the rat macrophages
Bachelor in Cell biology, Tehran University, Tehran, Iran, (1988-1993)
RESEARCH INTERESTS
I have broad interest in stem cell research related to physical conditions Just like magnetic fields, leading to differentiation and its application for Human diseases treatment, Regenerative medicine and Healthcare. Living systems including human brain involve moving charges, so response to magnetic fields. This is one of the great mysteries of live which can result in a safe and reliable way to cure human disorders.
PUBLICATIONS
a: Scientific articles
1. Sadri M., Rabbani O. Kouhian A. 2005 “Study of Laser Effect on Protein Synthesis and apoptosis in Alveolar Macrophages”; Modern Genetics No: 3 (32-38)
2. Sadri M., Rabbani O. Kouhian A. 2006 “Study of Laser on apoptosis Induction in the Alveolar Macrophages”; Kowsar Medical Jurnal vol11 No:2(143-154)
3. Maryam sadri, Parviz Abdolmale, Saeid Abroun, Bahareh Beiki, Fazel S. Samani. 2012 “Investigating the impacts of Static Magnetic Field on cell viability and Cell Cycle Progression in Human Mesenchymal Stem cells”; Iranian Joural of Biomedical engineering, Summer, vol.6, No2, pp: 91-98
4. Sadri M., Abdolmaleki P., Abrun S., Beiki B., Sahraneshin Samani F., 2017 "Static Magnetic Field Effect on Cell Alignment, Growth and Differentiation in Human cord-derived Mesenchymal Stem Cells" Cellular and Molecular Bioengineering, Vol. 10, No.3: 249–262
5. Maryam sadri, Parviz Abdolmaleki, Saeid Abroun, Mehrdad Behmanesh, Bahareh Beiki, 2018. Static Magnetic Field effect on Differentiation in Human Mesenchymal Stem Cells". J Stem Cells Res, Rev & Rep. 5(1): 1025.
b: Translation & Edition:
- Sadri M. Morovati S., Karami A. Genetics (2008), Published by Sepah education and training department (edited book)
- Earl Mindle’s Arthritis, (2004), Daneshgaran e Mahmoud press, Translated into Persian by Mariam Sadri
EXPERiMENTAL ACHIEVEMENTS
Attending the following:
- Theoretical course and participation in pluripotent Human Stem cell Culture and Expansion workshop (May 2010: Royan Institute-Tehran-Iran,www.royaninstitute.org)
3. Theoretical course: Nanotechnology Equipments; ( 2010 United Nations Association of Iran-Tehran, Tehran Iran)
4. Experimental skill courses; DNA & RNA extraction, Gel Electrophoresis, PCR
(Summer 1995: National Genetic & Biotechnology Research Institute-Tehran)
PROFESSIONAL SKILLS
- Rat alveolar macrophages extraction and culture (1995-1998)
- DNA extraction and DNA fragmentation estimation (1995-1998)
- Agarose Gel Electrophoresis
- Cell morphological studies and viability determination (1995-1998)
- RNA extraction &cDNA synthesis (2004-2010)
- Rt PCR (Revers Transcriptase Polymerase Chain Reaction (2004-2010)
- Umbilical Cord Blood-derived Hematopoietic stem cell culture and Expansion (2012-2014)
- Cell cycle progression analysis using flow cytometry (2013-2015)
- Human Umbilical Cord-derived mesenchymal stem cell isolation and expansion (2012-2014)
- Real time PCR (2014-2015)
EXPERIENCES
1. The examination of expansion and differentiation of human umbilical cord blood derived hematopoietic and mesenchymal stem cells as a Research Colleague 2010- 2011, Royan institute
2. Cell cycle & apoptosis analysis using flow cytometry Research Colleague 2011 Royan institute
3. Isolation and expansion of Wharton’s jelly derived mesenchyme stem cells
using an effective, rapid and economical method without any enzymatic treatment, as a Research Colleague 2011 to 2014
Royan cord blood ban
4. Polymerase chain reaction: Rt PCR and Real time PCR experiment as a Research Colleague 2013 to 2015 Tarbiat Modares University
TEACHING EXPERIENCES
University Course Date
Payamenour (Tehran-East) center Biophysics 2017-2018
Karaj Islamic Azad university Radiation Biology 2018 to date
Karaj Islamic Azad university Biophysics 2018 to date
PROFESSIONAL PRESENTATION
- Investigating the Impacts of Static Magnetic Field on cell Viability and Cell-Cycle Progression In Human Mesenchymal Stem Cells, 8th International Workshop on Biological Effects of EMF, 21-26 September 2014 Varna, Bulgaria
Dissertation abstract
Static Magnetic Field effect on Viability, Proliferation rate and Neural Differentiation in Umbilical Cord derived Human Mesenchymal Stem Cells
This investigation was performed to evaluate the impact of static magnetic field up to 24 miliTesla (24mT) impact on the viability percentage and the cell cycle progression in Mesenchymal Stem cells derived from human newborn cords. Cells from the third to fifth passage were harvested and counted to provide 60% confluent monolayers. The samples were then exposed to 6, 12, 18 and 24 mili-Tesla static magnetic field and the resulting samples were compared to the control none exposed cultures.
A significant (P<0.05) reduction of the cell viability due to the exposure of 24 hours of the magnetic field was noted in the time frames of 36 and 48 hours, However, the drop in stem cell viability percentage significantly leveled off in the exposed samples 72 hours after the exposure which may be due to cellular repair and regenerative mechanisms.
Development through the cell-cycle, also verified this finding: G1 phase cell population significantly decreased in the treated and cultured groups compared to the corresponding control samples. The reduced viable cell rates may be due to apoptosis since necrosis was never observed in our experiments.
In our experiments neural differentiation was enhanced in the 18 mT/24h static magnetic field exposed mesenchymal stem cells after 21 days of cultivation in differential medium. Immunocyto-chemical studies confirmed Nestin gene expression in the SMF treated samples. Furthermore, Oct-4, Nanong and Sox2 genes were less expressed while Heage-1 over expressed in the SMF exposed cell cultures which suggested the differentiation inducing effects of SMF exposure. Keywords:
Mesenchymal Stem Cell, Static Magnetic Field, Viability, Neural Differentiation